CD30 expression and Detection of Epstein-Barr Virus-Encoded Small RNA by Real-Time RT-PCR of Classic Hodgkin Lymphoma and Their Correlation with Risk Stratification

Abstract

More than 40% patient of Hodgkin lymphoma (HL) are chemoresistance and show early disease relapse that leads to poor prognostic. Epstein-Barr Virus (EBV) appears to have poor prognostic in HL. The CD30 expression at Hodgkin and Reed-Stenberg cells suggested has associated with advanced disease stage. The International Prognostic Score-7 (IPS) is a seven-parameter of risk stratification index for prognostic and as a consideration for treatment plan. The aim of this journal is to see correlation between EBV infection and CD30 expression with IPS-7 in Classic HL (CHL) patient.

Case-control research design was done to 40 Fixed Formalin Paraffin-Embedded (FFPE) of CHL at the Dr Hasan Sadikin Hospital, Bandung. Cases were 20 patients CHL of high-risk score IPS-7 and controls were 20 patients with low-risk score. Detection of EBV-encoded small RNA-1 (EBER1) by Real-Time RT-PCR and Immunohistochemical staining of CD30 was performed to all samples. All data were analyzed using Chi-Square test with p-value <0.05 of significant level.

EBV was present in 10 cases of CHL (25%). The result of this study shows a different statistically significant of EBV infection (p=0.028) and CD30 expression (p=0.004) between low risk and high-risk IPS-7 HL. CD30 expression (OR= 7.429) may provide stronger factor for risk assessment for the patient with HL, compare to EBER1 detection (OR=6.000).

This study concluded that CD30 expression and EBV Infection correlate with risk assessment IPS-7 in CHL.